5 Simple Statements About flow cytometers Explained
5 Simple Statements About flow cytometers Explained
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Information-enriched characterization of cell populations. Morphological facts from visuals can increase to the richness of flow cytometry info. One example is, the determine demonstrates an in any other case regular apoptosis assay applying Annexin V and PI, including mobile imaging to characterize cells in Each individual population to expose morphologically unique features. These insights couldn't happen to be obtained from multiplex staining alone.
As samples are acquired around the Attune CytPix Flow Cytometer, the significant-pace brightfield digicam captures and shops images of detected gatherings at a fee nearly six,000 pictures per second, depending upon the flow amount and graphic dimension. For greater overall flexibility, Attune Cytometric Computer software enables you to adjust the picture seize frequency as wanted and choose a certain gate for impression capture, if essential.
(B) RAW 264.seven macrophages in many cases are annotated inaccurately with regard to particle count because of their complexity and noncircular borders. The quantity of singlets obtainable for Assessment improves when the person trains and employs a far more exact design.
Learn new insights into your experimental details by visualizing populations employing mixed fluorescence and morphology facts. Our software program interface wizards help downsampling, concatenation (merged files) and dimensionality applications that happen to be developed in to the software for an intuitive, streamlined knowledge Examination practical experience.
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Below, a highly skilled user has gated singlets confidently. Soon after assessing the handbook singlet gate, the CytPix graphic-derived parameter ParticleCount reveals this gate is made up of much more than four% aggregates.
Pair your Attune flow cytometer with the autosampler for amplified workflow effectiveness, and increase robotic integration for max throughput.
Our scientists will design panels to suit your needs as often times you may need—gratis, no product obtain necessary.
Early indications of this contamination experienced at first been dismissed as particles. Backgating to discover new populations. Backgating imaged cells within the Attune CytPix Flow Cytometer also helps you to use morphological characteristics to discover intriguing subpopulations that could not be apparent from flow cytometry information by yourself.
People can look at function illustrations or photos in authentic-time to substantiate sample good quality, improve protocols, and file the morphology of each cell population recognized for Evaluation. Imaging capture also Gains from acoustic concentrating, which really helps to placement the cells to make sure that a pointy, centered graphic is attained.
AI-driven automatic picture Investigation Together with the Attune Cytometric Application interprets celebration capabilities into distinctive morphology parameters that can be coupled with normal fluorescence and scatter parameters to reveal hugely reproducible inhabitants changes and one of a kind subsets.
Software Be aware: Automatic graphic analysis lowers person-to-user variability in flow cytometry gating strategies
Leverage the strength of Highly developed engineering and innovation to accelerate your discoveries. Believe in in the Attune Flow Cytometer loved ones to provide the trustworthiness, pace, and accuracy that set your perform aside.
Adaptable payment system from Agilent Economic Alternatives assist you to have the essential instrumentation you will need for instant job, or to grow your lab.
At the center of each and every Attune Flow Cytometer lies our revolutionary acoustic concentrating engineering, which utilizes sound waves to meticulously align cells within the flow cell. This impressive technique surpasses common hydrodynamic approaches, resulting in a lot quicker processing speeds, Fantastic details good quality, and greater sample throughput.
The Attune Xenith cytometer is a large-parameter spectral analyzer, delivering get more info both of those spectral unmixing and traditional flow abilities for thorough and multipurpose cell Investigation.
The Attune CytPix Flow Cytometer will allow customers to visualize samples through acquisition, verify the accuracy of gating methods, and aid final results with reputable documentation for publication applications.
Below, we demonstrate that by gating methods employing graphic-centered quantitative parameters can distinguish interacting cells from coincident occasions to more precisely evaluate interacting cells.